EFEKTIVITAS KONSENTRASI ASAM ALKOHOL TERHADAP HASIL PEMERIKSAAN BASIL TAHAN ASAM (BTA) METODE ZIEHL NEELSEN

AZZAHRA, NABILA (2024) EFEKTIVITAS KONSENTRASI ASAM ALKOHOL TERHADAP HASIL PEMERIKSAAN BASIL TAHAN ASAM (BTA) METODE ZIEHL NEELSEN. Diploma thesis, Poltekkes Kemenkes Palembang.

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Abstract

Latar Belakang : Tuberkulosis (TB) adalah penyakit infeksi saluran pernapasan
yang disebabkan oleh infeksi Mycobacterium tuberculosis. Tujuan Penelitian :
Untuk mengetahui efektivitas konsentrasi asam alkohol terhadap hasil
pemeriksaan Basil Tahan Asam (BTA) metode Ziehl Neelsen. Penelitian ini
menggunakan variasi konsentrasi Asam Alkohol 1%, Asam Alkohol 3% sebagai
kontrol, Asam Alkohol 6%, dan Asam Alkohol 9%. Masing-masing perlakuan
tersebut di replikasi sebanyak 9 kali. Metode Penelitian : Penelitian ini bersifat
eksperimental laboratorium dengan desain post test only. Hasil Penelitian :
Pewarnaan BTA metode Ziehl Neelsen yang efektif yaitu pada konsentrasi asam
alkohol 3%, rata-rata skor pewarnaan BTA adalah 4.00 dengan skala minimum
4.00 dan skala maksimum 4.00, serta standar deviasi sebesar 0.00. Dari hasil
estimasi interval bahwa 95% diyakini rata-rata konsentrasi asam alkohol 3%
adalah antara 4.00-4.00. Kesimpulan : Konsentrasi asam alkohol 3% memiliki
efektivitas terhadap hasil pemeriksaan Basil Tahan Asam (BTA), karena sesuai
pedoman yang telah ditetapkan WHO.

Background: Tuberculosis (TB) is an infectious disease of the respiratory tract
caused by Mycobacterium tuberculosis infection. Research Objective: To
determine the effectiveness of alcoholic acid concentration on the results of acid
resistant bacilli (BTA) examination of the Ziehl Neelsen method. This study used
variations in the concentration of 1% alcoholic acid, 3% alcoholic acid as control,
6% alcoholic acid, and 9% alcoholic acid. Each treatment was replicated 9 times.
Research Methods: This research is a laboratory experiment with a post test only
design. Research Results: Effective BTA staining of Ziehl Neelsen method is at 3%
alcohol acid concentration, the average BTA staining score is 4.00 with a
minimum scale of 4.00 and a maximum scale of 4.00, and a standard deviation of
0.00. From the results of the interval estimation that 95% believed the average
concentration of 3% alcoholic acid was between 4.00-4.00. Conclusion: The
concentration of 3% alcoholic acid has effectiveness on the results of Acid
Resistant Bacilli (BTA) examination, because it is in accordance with the
guidelines set by WHO.

Item Type: Thesis (Diploma)
Uncontrolled Keywords: Basil Tahan Asam, Asam Alkohol
Subjects: R Medicine > RB Pathology
Divisions: Jurusan Teknologi Laboratorium Medis > 13350-D4 Teknologi Laboratorium Medis
Depositing User: Dibertha Aristi
Date Deposited: 06 Oct 2025 02:52
Last Modified: 06 Oct 2025 02:52
URI: http://rama.poltekkespalembang.ac.id/id/eprint/393

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