ANALISIS AKURASI DIAGNOSTIK METODE CARIK CELUP DENGAN METODE ASAM ASETAT 6% PADA PEMERIKSAAN PROTEIN URINE PASIEN SUSPEK PROTEINURIA DI PUSKESMAS SUGIH WARAS KABUPATEN OGAN KOMERING ILIR

Latar Belakang: Pemeriksaan protein urine sangat penting dilakukan, terutama pada populasi berisiko. Metode carik celup (dipstick) dan metode asam asetat 6% merupakan pemeriksaan protein urine yang sering digunakan di laboratorium, namun akurasi kedua metode tersebut masih menjadi pertanyaan karena belum banyak dilakukan uji akurasi diagnostik. Tujuan: Mengetahui gambaran akurasi diagnostik metode carik celup dengan metode asam asetat 6% pada pemeriksaan protein urine pasien suspek proteinuria di Puskesmas Sugih Waras Kabupaten Ogan Komering Ilir. Metode: Penelitian ini merupakan penelitian deskriptif observasional dengan pendekatan cross-sectional dilaksanakan pada bulan Juni 2026. Sampel berjumlah 63 urine pasien yang diambil dengan teknik purposive sampling dari populasi seluruh pasien yang melakukan pemeriksaan protein urine di laboratorium Puskesmas Sugih Waras. Analisis data dilakukan menggunakan uji diagnostik dengan tabel kontingensi 2x2. Hasil: Metode carik celup memiliki sensitivitas sebesar 70%, dan spesifisitas sebesar 88.68% dengan Akurasi sebesar 85.70%, NPP 53.50%,NPN sebesar 94.00%, LR+ sebesar 6.18 dan LR- sebesar 0.34. Metode asam asetat 6% memiliki sensitivitas sebesar 66.67%, spesifisitas sebesar 98.15%, akurasi sebesar 93.65%, NPP 85.70%, NPN sebesar 94.64%, LR+ sebesar 36.00 dan LR-sebesar 0.34. Kesimpulan: Terdapat perbedaan nilai akurasi diagnostik antara metode carik celup dan metode asam asetat 6% dimana metode asam asetat 6% memiliki nilai akurasi diagnostik yang lebih baik pada pemeriksaan protein urine pasien suspek proteinuria.

Background: Urine protein testing is essential, especially in at-risk populations. The dipstick method and the 6% acetic acid method are commonly used for urine protein testing in laboratories; however, the accuracy of both methods remains questionable because few diagnostic accuracy tests have been conducted. Objective: To determine the diagnostic accuracy of the dipstick method compared to the 6% acetic acid method in urine protein testing of patients with suspected proteinuria at the Sugih Waras Community Health Center, Ogan Komering Ilir Regency. Methods: This was a descriptive observational study with a cross-sectional design conducted in June 2026. The sample consisted of 63 urine specimens collected using purposive sampling from the entire population of patients who underwent urine protein testing at the Sugih Waras Community Health Center laboratory. Data analysis was performed using diagnostic tests with a 2x2 contingency table. Results: The dipstick method had a sensitivity of 70%, a specificity of 88.68%, an accuracy of 85.70%, a positive predictive value (PPV) of 53.50%, a negative predictive value (NPV) of 94.00%, a positive likelihood ratio (LR+) of 6.18, and a negative likelihood ratio (LR-) of 0.34. The 6% acetic acid method had a sensitivity of 66.67%, a specificity of 98.15%, an accuracy of 93.65%, a NPP of 85.70%, an NPN of 94.64%, a positive likelihood ratio (LR+) of 36.00, and a negative likelihood ratio (LR-) of 0.34. Conclusion: There is a difference in diagnostic accuracy between the dip-slip method and the 6% acetic acid method, with the 6% acetic acid method having higher diagnostic accuracy.